Heterogeneous LPS of Porphyromonas gingivalis differentially modulate the innate immune response of human gingiva
نویسندگان
چکیده
Methods Reconstituted human gingival epithelia (RHGE) were challenged with two isoforms of PgLPS together with E. coli LPS as the positive control. mRNA and proteins were harvested from tissues and culture supernatants were collected. Expression of pro-inflammatory and antiinflammatory cytokines was evaluated by Q-PCR and ELISA. Involvement of pattern recognition receptors and signaling pathways were also analyzed by Q-PCR and western blot. Next, RHGE was blocked for CD14, TLR2, and TLR4 and followed by stimulation of PgLPS isoforms and effect was evaluated at cytokine level by Q-PCR and ELISA. Furthermore, we used “tissue proteomics” approach to study the differential proteomic expression profiles of gingival epithelium upon Pg LPS stimulation.
منابع مشابه
Heterogeneous Porphyromonas gingivalis LPS modulates immuno-inflammatory response, antioxidant defense and cytoskeletal dynamics in human gingival fibroblasts
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